TAKARA双酶切表
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1、Enzyme Acc\ BamH I eg/II Cla\ EcoR 1 EcoR V Hine II Hind III Kpn\ Neo I Ndel Acc\ 1xM 0.5 xK 1xT 1xM 1xM 0.5 xK 1XM 1xM 1xM IxM+BSA 1xT BamH 1 0.5 xK 1xK 1xK 1xK 1xK 1xK 0.5 xK 1XK 0.5 xK IXK+BSA 1xK Bglll 1xT 1XK 1xH 1XH 1xH 1xH 2xK 1xK 1XT 1XK.8SA 1xH
2、 C/al 1xM 1xK 1xH 1xM 1xH 1XH 1xM 1xM 1xM IxK+BSA 1xH EcoR I 1XM 1xK 1xH 1xH 1xH 1xH 1xM 1xM 1xM IxKiBSA 1xH EcoR V 0.5 xK 1xK 1xH 1xH 1xH 1xH 2xT 1xK 0.5 xK IxK+BSA 1xH Hincl\ 1xM 0.5 xK 2xK 1xM 1xM 2xT 1XM 1xM 1xM 1XM+BSA 1xT Hind 111 1xM 1xK 1xK
3、 1xM 1xM 1xK 1xM 1xM 1xM IxK+BSA 1xK Kpn\ 1xM 0.5 xK 1XT 1xM 1xM 0.5 xK 1xM 1xM 1xL 0.5XK+BSA 1xT Nco\ ixMiBSA IxKiBSA IxKiBSA ixKiBSA IxK+BSA ixKiBSA IxMiBSA IxKfBSA 0.5xKi8SA IxKiBSA IxK+BSA Nde\ 1xT 1xK 1xH 1xH 1xH 1xH IxT 1xK 1xT IxK+BSA 1XH Noti 0.5X
4、K+BSA 0.5xKiBSA IxHiBSA IxlUBSA IxHiBSA 1xHi8SA 0.5xKiBSA 05XK.BSA 05xKf8SA 05xK*BSA IxH+BSA Pst\ 1xM 1xK 1xH 1xH 1xH 1xH 1xM 1xM 1xM ixKtBSA 1xH Pvul 0.5 xK 1xK 1xK 1xK 1xK 1xK 0.5 xK 1XK 0.5 xK ixKtBSA 1xK Sac\ 1xM 0.5 xK 0.5 xK 1xM 1XM 0.5 xK 1xM 1xM 1X
5、L 0.5xKi8SA 1xT Sail 1.5xT 1.5XT 1xH 1xH 1xH 1xH 1.5xK 1.5xK UxW 15XUBSA 1xH Smal IxTiBSA 0.5xTfBSA ixTiBSA IxTiBSA IxTiBSA 0.5XK+BSA 1XT+BSA IxTiBSA IxTiBSA lxb8SA IxT+BSA Spel 1xM 1xK 1xH 1xM 1xH 1xH 1xM 1XM 1xM IxKiBSA 1xH Sphl 0.5 xK 1xK 1xH 1xH 1xH 1
6、xH 2xT 1xK 0.5 xK IxKtBSA 1xH Xba\ 1 xM 0.5 xK 2xT 1xM 1xM 2xT 1xM 1XM 1xM IxM+BSA 1xT Xho\ 1xM 1xK 1xH 1xH 1xH 1xH 1xM 1xM 1xM IXK+BSA 1xH En zyme Not l Pstl Pvul Sacl Sa/I Smal Spel Sph 1 Xbal Xho\ Accl 0.5XK+BSA 1xM 0.5 xK 1xM 1.5xT 1 xT+BSA 1xM
7、 0.5 xK 1xM 1xM BamH 1 0.5XK+BSA 1XK 1xK 0.5 xK 1.5xT 0.5XT+BSA 1xK 1xK 0.5 xK 1xK Bglll 1XH+BSA 1XH 1XK 0.5 xK 1XH 1 xT+BSA 1xH 1xH 2xT 1xH Clal IxH+BSA 1xH 1xK 1xM 1xH 1 xT+BSA 1xM 1xH 1xM 1xH EcoR 1 1XH+BSA 1xH 1xK 1xM 1xH 1 xT+BSA 1xH 1xH 1xM 1xH Ec
8、oR V 1XH+BSA 1xH 1xK 0.5 xK 1xH 0.5XK+BSA 1xH 1xH 2xT 1xH Hine 11 0.5XK+BSA 1xM 0.5 xK 1xM 1.5xK 1 xT+BSA 1XM 2xT 1xM 1xM Hind III 0.5XK+BSA 1xM 1xK 1xM 1.5xK 1 xT+BSA 1xM 1xK 1xM 1xM Kpn 1 0.5XK+BSA 1xM 0.5 XK 1xL 1.5XT+BSA 1 xT+BSA 1xM 0.5 xK 1xM 1xM Neo
9、 1 0.5XK+BSA 1XK+BSA IxK+BSA Q.5XK+BSA 1.5XT+BSA 1 xT+BSA 1XK+BSA 1XK+BSA IxM+BSA IxK+BSA Ndel IxHfBSA 1XH 1xK 1xT 1xH 1 xT+BSA 1xH 1xH 1xT 1xH Not I IxH+BSA 用onX-100 1XH+BSA 2XK+BSA 0.5XK+BSA IxH+BSA 0.5xTiBSA IxH+BSA IxH+BSA 0.5XK+BSA IxH+BSA Pstl 1XH+BSA 1xH 1x
10、K 1xM 1xH 0.5xTiBSA 1xH 1xH 1XM 1xH Pvul 2XK+BSA 1XK 1xK +BSA 0.5 xK L5XK+BSA 1XK+BSA 1xK 1xK 0.5 xK 1xK Sacl 0.5XK+BSA 1xM 0.5 xK 1xL 1.5XT+BSA 1 xT+BSA 1xM 0.5 xK 1xM 1xM Sall IxH+BSA 1xH 1.5XK+BSA 1.5XT+BSA 1xH 1.5XT+8SA 1xH 1xH 1.5xT 1xH Smal 0.5XT+BSA
11、0.5XT+BSA IxK+BSA IxT+BSA 1.5XT+BSA 1 xT+BSA 1 xT+BSA 0.5XT+BSA 1 xT+BSA 1 xT+BSA Spel 1XH+BSA 1XH 1xK 1xM 1xH 1 xT+BSA 1xM 1xH 1xM 1xH Sphl 1XH+BSA 1xH 1xK 0.5 xK 1xH 0.5xTiBSA 1xH 1xH 2xT 1xH Xbal 0.5XK+BSA 1xM 0.5 xK 1XM 1.5XT 1 xT+BSA 1xM 2xT IxM+BSA 1xM Xhol IXH+BSA 1xH 1xK 1xM 1xH 1 xT+BSA 1xH 1xH 1xM 1xH
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