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1、0 Invitrogen
life technologies
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Comments fo
2、r pcDNA3.1 什) 5428 nucleotides
CMV promoter: bases 232-819
T7 promoter/prim ing site: bases 863-882
Multiple clo ning site: bases 895-1010
pcDNA3.1/BGH reverse primi ng site: bases 1022-1039 rUC
BGH polyade nylation seque nee: bases 1028-1
3、252
f1 origi n: bases 1298-1726
SV40 early promoter and origin: bases 1731-2074
Neomycin resista nee gene (ORF): bases 2136-2930
SV40 early polyade nylation sig nal: bases 3104-3234
pUC origi n: bases 3617-4287 (compleme ntary stra nd)
Ampicillin resista nee gene ( bla): bases 4432-5428 (compleme ntary stra nd)
ORF: bases 4432-5292 (compleme ntary stra nd)
Ribosome binding site: bases 5300-5304 (compleme ntary stra nd)
bla promoter (P3): bases 5327-5333 (compleme ntary stra nd)
pcdna3.1+图谱
